DENND1B Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD218656A
Western blot analysis of Human fetal brain tissue and mouse brain tissue lysates using DENND1B Polyclonal Antibody at dilution of 1:400
Product Name
DENND1B Polyclonal Antibody
Catalog Number
RD218656A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery.
Immunogen Information
Immunogen
Fusion protein of human DENND1B
Swissprot
Q6P3S1
Synonyms
C1ORF18C1orf218Chromosome 1 open reading frame 218Connecdenn 2DEN1BDENN domain containing protein 1BDENN domain-containing protein 1BDENN MADD domain containing 1BDENND1BDKFZp547O0715FAM31BFamily with sequence similarity 31 member BFLJ20054
Calculated MW
86 kDa
Observed MW
Refer to figures
Gene Accession
BC063877
Applications
Reactivity
Human, Mouse
Tested Applications
WB,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, ELISA 1:5000-1:10000
Concentration
0.54 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas