DHFR Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD252839A
Western blot analysis of 293T cell lysate using DHFR Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Human tonsil tissue using DHFR Polyclonal Antibody at dilution of 1:50(×200)
Product Name
DHFR Polyclonal Antibody
Catalog Number
RD252839A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
Fusion protein of human DHFR
Swissprot
P00374
Synonyms
DHFRDHFRP1Dihydrofolate reductaseDYRDYREC 1.5.1.3
Calculated MW
21 kDa
Observed MW
Refer to figures
Gene Accession
BC000192
Applications
Reactivity
Human, Mouse, Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:5000-1:10000
Concentration
0.96 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas