Deoxyribonuclease I (DNase I) is an endonuclease which is secreted to cleave DNA in the extracellular space down to an average of tetranucleotides with 5′ monophosphate and 3′ hydroxyl DNA ends . Both single-stranded DNA and double-stranded DNA are degraded by Dnase I. This nuclease appears to account for the major nucleolytic activity on DNA in serum and is responsible for the degradation of the majority of circulating DNA derived from apoptotic and necrotic cell death and from neutrophil extracellular traps. In addition to its role in the serum, it has been proposed as one of the deoxyribonucleases responsible for DNA fragmentation in the process of apoptosis.
Immunogen Information
Immunogen
Recombinant Human DnaseⅠ protein expressed by E.coli.
Gene ID
1773
Swissprot
P24855
Synonyms
Deoxyribonuclease-1DNASE1DNL1DRNI
Calculated MW
15kDa
Observed MW
23kDa
Applications
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
0.7mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.