DNMT1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90294A
Western blot analysis of extracts of various cell lines using DNMT1 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of 293T cells using DNMT1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug DNMT1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using DNMT1 at a dilution of 1:1000.
Product Name
DNMT1 Polyclonal Antibody
Catalog Number
RD90294A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human DNMT1
Gene ID
1786
Swissprot
P26358
Synonyms
DNMT1ADCADNAIMCXXC9DNMTHSN1EMCMTm.HsaIDNA methyltransferase 1
Calculated MW
12kDa
Observed MW
Refer to figures
Applications
Reactivity
Human
Tested Applications
WB,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas