DOK3 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD252149A
Western blot analysis of Human notum skin cancer tissue using DOK3 Polyclonal Antibody at dilution of 1:500
Product Name
DOK3 Polyclonal Antibody
Catalog Number
RD252149A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
DOK3 gene maps to chromosome 5q35.3. Dok3 was tyrosine phosphorylated by Src family members Lck, Fyn, and Lyn. Immunoprecipitation studies showed that Dok3 bound inhibitors SHIP and Csk but did not bind RasGAP. Dok3 binding to SHIP occurred via the SH2 domain. Dok3 also bound Csk via the Csk SH2 domain with possible involvement of the Csk SH3 domain as well.DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function
Immunogen Information
Immunogen
Fusion protein of human DOK3
Swissprot
Q7L591
Synonyms
Docking protein 3DOK 3DOK Like ProteinDok3DOK3DOKL PendingDownstream of tyrosine kinase 3p62 DOK Like Protein
Calculated MW
53 kDa
Observed MW
Refer to figures
Gene Accession
BC004564
Applications
Reactivity
Human
Tested Applications
WB,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, ELISA 1:1000-1:5000
Concentration
0.4 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas