EEA1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD80086A
Western blot analysis of extracts of various cell lines using EEA1 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat lung using EEA1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human mammary cancer using EEA1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using EEA1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Product Name
EEA1 Polyclonal Antibody
Catalog Number
RD80086A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector,mediates endosome docking and,together with SNAREs,leads to membrane fusion.
Immunogen Information
Immunogen
Recombinant fusion protein of human EEA1 (NP_003557.2).
Gene ID
8411
Swissprot
Q15075
Synonyms
EEA1MST105MSTP105ZFYVE2
Calculated MW
162 kDa
Observed MW
175 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas