EED Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD89669A
Western blot analysis of extracts of various cell lines using EED Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using EED Polyclonal Antibody at 1:1000 dilution.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug EED Polyclonal Antibody.Western blot was performed from the immunoprecipitate using EED Polyclonal Antibody at a dilution of 1:1000.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug EED Polyclonal Antibody.Western blot was performed from the immunoprecipitate using EED Polyclonal Antibody at a dilution of 1:1000.
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Overview

Circle
Product Name
EED Polyclonal Antibody
Catalog Number
RD89669A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human EED
Gene ID
8726
Swissprot
O75530
Synonyms
EEDHEEDWAIT1
Calculated MW
24kDa
Observed MW
25kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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