EEF1E1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot analysis of extracts of various cell lines using EEF1E1 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of L929 cells using EEF1E1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
EEF1E1 Polyclonal Antibody
Catalog Number
RD82097A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2.
Immunogen Information
Immunogen
A synthetic peptide of human EEF1E1 (NP_004271.1).
Gene ID
9521
Swissprot
O43324
Synonyms
EEF1E1AIMP3P18
Calculated MW
15 kDa/19 kDa
Observed MW
20 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:200-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas