EGLN1 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD82623A
Immunofluorescence analysis of MCF-7 cells using EGLN1 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using EGLN1 Polyclonal Antibody
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Overview

Circle
Product Name
EGLN1 Polyclonal Antibody
Catalog Number
RD82623A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human EGLN1 (NP_071334.1).
Gene ID
54583
Swissprot
Q9GZT9
Synonyms
EGLN1C1orf12ECYT3HALAHHIF-PH2HIFPH2HPH-2HPH2PHD2SM20ZMYND6

Applications

Circle
Reactivity
Human
Tested Applications
IF
Conjugation
Unconjugated
Dilution
IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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