For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87784A
Western blot analysis of extracts of various cell lines using EIF2AK1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human liver using EIF2AK1 Polyclonal Antibody at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human EIF2AK1
Gene ID
27102
Swissprot
Q9BQI3
Synonyms
EIF2AK1HCRHRI
Calculated MW
32kDa/33kDa/37kDa
Observed MW
38kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.