EIF3J Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot analysis of extracts of various cell lines using EIF3J Polyclonal Antibody at dilution of 1:3000.
Immunohistochemistry of paraffin-embedded Rat brain using EIF3J Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung using EIF3J Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human gastric cancer using EIF3J Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse spleen using EIF3J Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using EIF3J Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using EIF3J Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using EIF3J Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
EIF3J Polyclonal Antibody
Catalog Number
RD85221A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen Information
Immunogen
Recombinant fusion protein of human EIF3J (NP_003749.2).
Gene ID
8669
Swissprot
O75822
Synonyms
EIF3JEIF3S1eIF3-alphaeIF3-p35
Calculated MW
22 kDa/23 kDa/29 kDa
Observed MW
37 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:200-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas