eIF4A Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89240A
Western blot analysis of extracts of various cell lines using eIF4A Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of C6 cells using eIF4A Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using eIF4A Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using eIF4A Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
eIF4A Polyclonal Antibody
Catalog Number
RD89240A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Immunogen Information
Immunogen
A synthetic peptide of human eIF4A
Gene ID
1973/1974/9775
Swissprot
P60842/Q14240/P38919
Calculated MW
41kDa/43kDa
Observed MW
50KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.