EIF4A1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85516A
Western Blot analysis of 1) 293T, 2) Hela, 3) HepG2, 4) Mouse brain with eIF4A1 Monoclonal Antibody.
Immunohistochemistry of paraffin-embedded Mouse colon tissue using eIF4A1 Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Mouse spleen tissue using eIF4A1 Monoclonal Antibody at dilution of 1:200.
Product Name
EIF4A1 Monoclonal Antibody
Catalog Number
RD85516A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
EIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Immunogen Information
Immunogen
Synthetic Peptide
Swissprot
P60842
Synonyms
ATP-dependent RNA helicase eIF4A-1DDX2DDX2AeIF 4A IeIF-4A-IEIF4AeIF4A IeIF4A-IEIF4A1Eukaryotic initiation factor 4A-IEukaryotic initiation factor 4AIeukaryotic translation initiation factor 4Aisoform 1Eukaryotic translation initiation factor
Observed MW
48 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:3000, IHC 1:50-1:300, IF 1:100-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas