eIF4EBP1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90295A
Immunofluorescence analysis of U2OS cells using eIF4EBP1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
eIF4EBP1 Polyclonal Antibody
Catalog Number
RD90295A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Immunogen Information
Immunogen
Recombinant fusion protein of human Eif4ebp1
Gene ID
1978
Swissprot
Q13541
Synonyms
EIF4EBP14E-BP14EBP1BP-1PHAS-Ieukaryotic translation initiation factor 4E-binding protein 1
Calculated MW
48kDa/55kDa
Observed MW
60KDa
Applications
Reactivity
Human
Tested Applications
IF
Conjugation
Unconjugated
Dilution
IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas