EIF5B Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD266431A
Western blot analysis of extracts of various cell lines using EIF5B Polyclonal Antibody.
Immunofluorescence analysis of C6 cells using EIF5B Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using EIF5B Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using EIF5B Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
EIF5B Polyclonal Antibody
Catalog Number
RD266431A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.
Immunogen Information
Immunogen
Recombinant fusion protein of human EIF5B (NP_056988.3).
Gene ID
9669
Swissprot
O60841
Synonyms
EIF5BIF2
Calculated MW
138 kDa
Observed MW
179 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas