EIF6 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD82347A
Western blot analysis of extracts of various cell lines using EIF6 Polyclonal Antibody at dilution of 1:1000.
Confocal immunofluorescence analysis of Hela cells using EIF6 Polyclonal Antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2OS cells using EIF6 Polyclonal Antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of MCF-7 cells using EIF6 Polyclonal Antibody
Product Name
EIF6 Polyclonal Antibody
Catalog Number
RD82347A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human EIF6 (NP_852133.1).
Gene ID
3692
Swissprot
P56537
Synonyms
EIF6CABEIF3AITGB4BPb(2)gcneIF-6p27(BBP)p27BBP
Calculated MW
23 kDa/26 kDa
Observed MW
27 kDa
Applications
Reactivity
Human
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas