EN2 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD217909A
Western blot analysis of Jurkat cell lysate  using EN2 Polyclonal Antibody at dilution of 1:400
Western blot analysis of Jurkat cell lysate using EN2 Polyclonal Antibody at dilution of 1:400
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Overview

Circle
Product Name
EN2 Polyclonal Antibody
Catalog Number
RD217909A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Immunogen Information

Circle
Immunogen
Synthetic peptide of human EN2
Swissprot
P19622
Synonyms
AUTS 1AUTS1EN 2Engrailed 2Engrailed homeo box 2Engrailed homeobox 2Engrailed homolog 2Engrailed2Homeobox protein engrailed 2Homeobox protein engrailed2Hu En 2HuEn2MabEn
Calculated MW
34 kDa
Observed MW
Refer to figures
Gene Accession
NP001418

Applications

Circle
Reactivity
Human, Mouse
Tested Applications
WB,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, ELISA 1:5000-1:10000
Concentration
0.96 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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