ENO3 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD252747A
Western blot analysis of HepG2 NIH/3T3 C2CL2 Raji and Jurkat cell lysates  using ENO3 Polyclonal Antibody at dilution of 1:1000
Western blot analysis of HepG2 NIH/3T3 C2CL2 Raji and Jurkat cell lysates using ENO3 Polyclonal Antibody at dilution of 1:1000
Immunohistochemistry of paraffin-embedded Human tonsil tissue  using ENO3 Polyclonal Antibody at dilution of 1:70(×200)
Immunohistochemistry of paraffin-embedded Human tonsil tissue using ENO3 Polyclonal Antibody at dilution of 1:70(×200)
Immunohistochemistry of paraffin-embedded Human brain tissue  using ENO3 Polyclonal Antibody at dilution of 1:70(×200)
Immunohistochemistry of paraffin-embedded Human brain tissue using ENO3 Polyclonal Antibody at dilution of 1:70(×200)
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Overview

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Product Name
ENO3 Polyclonal Antibody
Catalog Number
RD252747A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit

Background

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This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.

Immunogen Information

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Immunogen
Fusion protein of human ENO3
Swissprot
P13929
Synonyms
2 phospho D glycerate hydrol yase2 phospho D glycerate hydrolyase2-phospho-D-glycerate hydro-lyaseBeta enolaseBeta-enolaseENO3ENOBEnolase 3 (beta muscle)Enolase 3Enolase3GSD13MSEMuscle specific enolaseMuscle-specific enolaseSkeletal muscle e
Calculated MW
47 kDa
Observed MW
Refer to figures
Gene Accession
BC017249

Applications

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Reactivity
Human, Mouse, Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-1:200, ELISA 1:5000-1:10000
Concentration
1.26 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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