ERK 1/2 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85563A
Western Blot analysis of 1) Hela, 2)Mouse brain, 3) Rat brain using ERK 1/2 Monoclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human colon carcinoma tissue using ERK 1/2 Monoclonal Antibody at dilution of 1:200.
Product Name
ERK 1/2 Monoclonal Antibody
Catalog Number
RD85563A
Clonality
Monoclonal
Purification Method
Protein A purification
Isotype
IgG
Host
Mouse
Background
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
Immunogen Information
Immunogen
Synthetic Peptide of ERK1/2
Swissprot
P27361,P28482
Synonyms
MAPK3ERK1PRKM3Mitogen-activated protein kinase 3MAP kinase 3MAPK 3ERT2Extracellular signal-regulated kinase 1ERK-1Insulin-stimulated MAP2 kinaseMAP kinase isoform p44p44-MAPKMicrotubule-associated protein 2 kinasep
Observed MW
44,42 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p
Conjugation
Unconjugated
Dilution
WB 1:1000-2000, IHC 1:100-200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas