ESPL1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD76827A
Western Blot analysis of Raji cell using ESPL1 Polyclonal Antibody at dilution of 1:500
Immunohistochemistry of paraffin-embedded Human thyroid cancer using ESPL1 Polyclonal Antibody at dilution of 1:40
Immunohistochemistry of paraffin-embedded Human cervical cancer using ESPL1 Polyclonal Antibody at dilution of 1:40
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
ESPL1 Polyclonal Antibody
Catalog Number
RD76827A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 or STAG2 in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 by ESPL1, or separase, which initiates the final separation of sister chromatids.
Immunogen Information
Immunogen
Synthetic peptide of human ESPL1
Swissprot
Q14674
Synonyms
Caspase like protein ESPL1Caspase-like protein ESPL1ESP 1ESP1ESPL 1Espl1ESPL1Extra spindle poles like 1Extra spindle poles like 1 proteinExtra spindle poles-like 1 proteinFLJ46492KIAA0165SeparaseSeparinSimilar to fission yeast cut1and geneS
Calculated MW
233 kDa
Gene Accession
NP_036423
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:200-1:1000, IHC 1:25-1:100
Concentration
0.5 mg/mL
Storage Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas