For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86805A
Western blot analysis of extracts of various cell lines using EXO1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human esophageal cancer using EXO1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human esophagus using EXO1 Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms.
Immunogen Information
Immunogen
Recombinant fusion protein of human EXO1
Gene ID
9156
Swissprot
Q9UQ84
Synonyms
EXO1HEX1hExoI
Calculated MW
51kDa
Observed MW
51KDa
Applications
Reactivity
Human
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.