EYA3 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD266408A
Western blot analysis of extracts of various cell lines using EYA3 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of U2OS cells using EYA3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
EYA3 Polyclonal Antibody
Catalog Number
RD266408A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human EYA3 (NP_001981.2).
Gene ID
2140
Swissprot
Q99504
Synonyms
EYA3
Calculated MW
48 kDa/56 kDa/57 kDa/58 kDa/62 kDa
Observed MW
57 kDa/58 kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.