EZH2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD70338A
Western Blot analysis of various samples using EZH2 Polyclonal Antibody at dilution of 1:500.
Product Name
EZH2 Polyclonal Antibody
Catalog Number
RD70338A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
EZH2 (enhancer of zeste homologue 2,also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation,and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors,including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635,21367748)
Immunogen Information
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse EZH2
Swissprot
Q15910,Q61188
Synonyms
Enhancer of zeste 2enhancer of zeste 2 polycomb repressive complex 2 subunitEnhancer of zeste homolog 2 (Drosophila)Enhancer of zeste homolog 2Enhancer of zesteDrosophilahomolog 2ENX 1Enx 1hENX-1ENX1Enx1hEZH 2EZH1EZH2EZH2EZH2bHistone-lysi
Calculated MW
80-100 kDa
Observed MW
80 kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
0.54 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas