F11R Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using JAM-A/CD321/F11R Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using JAM-A/CD321/F11R Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using JAM-A/CD321/F11R Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using JAM-A/CD321/F11R Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using JAM-A/CD321/F11R Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat brain using JAM-A/CD321/F11R Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human placenta using JAM-A/CD321/F11R Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human placenta using JAM-A/CD321/F11R Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Overview

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Product Name
F11R Polyclonal Antibody
Catalog Number
RD86759A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.

Immunogen Information

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Immunogen
Recombinant fusion protein of human F11R
Gene ID
50848
Swissprot
Q9Y624
Synonyms
F11RCD321JAMJAM1JAMAJCAMKATPAM-1
Calculated MW
68kDa
Observed MW
69KDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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