F4/80 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD90247A
Western blot analysis of various lysates using F4/80 Polyclonal Antibody at 1:500 dilution.
Western blot analysis of various lysates using F4/80 Polyclonal Antibody at 1:500 dilution.
Immunofluorescence analysis of RAW264.7 cells using F4/80 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of RAW264.7 cells using F4/80 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
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Overview
Circle
Product Name
F4/80 Polyclonal Antibody
Catalog Number
RD90247A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Circle
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg development.
Immunogen Information
Circle
Immunogen
Recombinant fusion protein of mouse F4/80
Gene ID
13733
Swissprot
Q61549
Synonyms
DD7A5-7EGF-TM7Emr1F4/80Gpf480Ly71TM7LN3
Calculated MW
48kDa/52kDa/25kDa/35kDa/43kDa
Observed MW
52kDa/60kDa
Applications
Circle
Reactivity
Human,Mouse
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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