FADD Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90260A
Western blot analysis of extracts of Mouse heart using FADD Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Jurkat cells using FADD Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of mouse bone marrow cells using FADD Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600ug extracts of Mouse heart cells using 3ug FADD Polyclonal Antibody. Western blot was performed from the immunoprecipitate using FADD Polyclonal Antibody at a dilution of 1:1000.
Product Name
FADD Polyclonal Antibody
Catalog Number
RD90260A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95 or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.
Immunogen Information
Immunogen
Recombinant fusion protein of mouse FADD
Gene ID
14082
Swissprot
Q61160
Synonyms
Mort1/FMort1/FADD
Observed MW
46-75KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:200-1:2000,IF 1:50-1:200,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.