For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87971A
Western blot analysis of extracts of various cell lines using FIP200 Polyclonal Antibody at1:1000 dilution.
Western blot analysis of extracts of various cell lines using FIP200 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human esophageal cancer using FIP200 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Immunogen Information
Immunogen
Recombinant fusion protein of human FIP200
Gene ID
9821
Swissprot
Q8TDY2
Synonyms
RB1CC1ATG17CC1FIP200PPP1R131
Calculated MW
125kDa
Observed MW
130kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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