Flag-Tag Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86725A
Western blotting with anti-FLAG monoclonal antibody at dilution of 1:5000.lane 1: HEK 293 whole cell lysate, Lane 2: FLAG tag transfected HEK 293 cell lysates
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-Flag-Tag Monoclonal Antibody at 1:2000 dilution.
Lane 1:HEK-293 transfected with DDDDK-tagged (input).Lane 2: Mouse monoclonal IgG2a instead of RD86725A in HEK-293T transfected with DDDDK-tagged.Lane 3: RD86725A IP in HEK-293T transfected with DDDDK-tagged.
1x10^6 CHO cells Transfected with a Flag plasmid were stained with 0.2ug Anti-Flag tag mAb(RD86725A) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
Product Name
Flag-Tag Monoclonal Antibody
Catalog Number
RD86725A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG2a
Host
Mouse
Background
FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications.
Immunogen Information
Immunogen
Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin.
Synonyms
DDDDK epitope tagDDDDK epitope tagDYKDDDDK epitope tag
Applications
Reactivity
All
Tested Applications
WB, IF,IP,FCM
Conjugation
Unconjugated
Dilution
WB 1:5000-1:10000, IF 1:500-1:2000,IP 3ug/sample,FCM:0.2 ug
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.