Flag-Tag Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86633A
Western blotting with Anti-FLAG rabbit polyclonal antibody at dilution of 1:1000.Lane1: FLAG tag transfected HEK 293 whole cell lysate, Lane2: HEK 293 whole cell lysate
Immunofluorescent analysis of Hela cells transfected with the plasmid overexpressing Flag fusion Protein, using anti-Flag -Tag polyclonal antibody at 1:200 dilution.
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-Flag-Tag Polyclonal Antibody at dilution of 1:12000.
IP Result of 293F cells transfected with FLAG-Tag fusion protein,using anti-FLAG-Tag rabbit antibody . Lane 1:input, lane 2: rabbit IgG Isotype Control, Lane 3: anti-FLAG-Tag rabbit antibody
Variation
Quantity
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Overview
Product Name
Flag-Tag Polyclonal Antibody
Catalog Number
RD86633A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This
Immunogen Information
Immunogen
Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin.
Synonyms
DDDDK epitope tagDDDDK epitope tag
Calculated MW
26kDa
Observed MW
26kDa
Applications
Reactivity
All
Tested Applications
WB, IF,IP
Conjugation
Unconjugated
Dilution
WB 1:2000-1:5000, IP 3ug/sample, IF 1:6000-1:12000
Concentration
4mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.