FOS Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90215A
Western blot analysis of extracts of various cell lines using FOS Polyclonal Antibody at 1:1000 dilution.NIH/3T3 cells were treated by Serum-starvation overnight at 37℃.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Variation
Quantity
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Overview
Product Name
FOS Polyclonal Antibody
Catalog Number
RD90215A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Immunogen Information
Immunogen
A synthetic peptide of human FOS
Gene ID
2353
Swissprot
P01100
Synonyms
FOSAP-1C-FOSp55c-Fos
Calculated MW
85kDa/155kDa/157kDa
Observed MW
170KDa
Applications
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas