FOXO1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD70350A
Western Blot analysis of various samples using FOXO1 Polyclonal Antibody at dilution of 1:1000.
Product Name
FOXO1 Polyclonal Antibody
Catalog Number
RD70350A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
FOXO1,also named as FOXO1A,FKHR and FKH1,is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1,FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24,Ser-256,and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).
Immunogen Information
Immunogen
Recombinant protein corresponding to Mouse FOXO1
Swissprot
Q9R1E0,G3V7R4
Synonyms
FKH 1FKH1FKHRForkhead (Drosophila) homolog 1 (rhabdomyosarcoma)Forkhead box O1Forkhead box protein O1Forkhead box protein O1AForkhead in rhabdomyosarcomaForkheadDrosophilahomolog ofin rhabdomyosarcomaFoxO transcription factorfoxo1FOXO1FOXO1
Calculated MW
78-82 kDa
Observed MW
78 kDa
Applications
Reactivity
Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
0.58 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.