FZD5 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87530A
Western blot analysis of extracts of rat kidney using FZD5 Polyclonal Antibody at 1:3000 dilution.
Immunohistochemistry of paraffin-embedded human colon using FZD5 Polyclonal Antibody at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using FZD5 Polyclonal Antibody at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Variation
Quantity
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Overview
Product Name
FZD5 Polyclonal Antibody
Catalog Number
RD87530A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand.
Immunogen Information
Immunogen
Recombinant fusion protein of human FZD5
Gene ID
7855
Swissprot
Q13467
Synonyms
FZD5C2orf31HFZ5
Calculated MW
43kDa
Observed MW
44KDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.