For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89010A
Western blot analysis of extracts of various cell lines using GABRA2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse stomach using GABRA2 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human GABRA2
Gene ID
2555
Swissprot
P47869
Synonyms
GABRA2
Calculated MW
29kDa
Observed MW
28KDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.