Galectin 3 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90645A
Western Blot analysis of 1)MCF-7, 2)NIH/3T3, 3)Raw264.7 using Galectin 3 Ployclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human colon cancer using LGALS3 Polyclonal Antibody at dilution of 1:300
Immunohistochemistry of paraffin-embedded Human stomach cancer using LGALS3 Polyclonal Antibody at dilution of 1:300
Product Name
Galectin 3 Polyclonal Antibody
Catalog Number
RD90645A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides . Galectin-3 contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers. Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell,being present on the cell surface or in the extracellular space . Galectin-3 is involved in various biological processes including cell growth,adhesion,differentiation,apoptosis,angiogenesis,immune response,neoplastic transformation and metastasis .
Immunogen Information
Immunogen
Recombinant Human LGALS3 protein expressed by E.coli
Swissprot
P17931
Synonyms
35 kDa lectinCarbohydrate binding protein 35Carbohydrate-binding protein 35CBP 35CBP35Gal-3GAL3Galactose-specific lectin 3Galactoside-binding proteinGALBPGalectin 3 internal geneincludedGalectin-3Galectin3GALIGGBPIgE binding proteinIgE-bi
Calculated MW
10 kDa
Observed MW
10 kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:150-1:300
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.