GAPDH Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western Blot analysis of GAPDH in various cell lines at dilution of 1:5000.
Lane1: DU145 whole cell lysate, Lane2: OS-RC-2 whole cell lysate, Lane3:T-47D whole cell Lysates, Lane4: HEC-1B whole cell lysate, Lane5: HepG2 whole cell lysate, Lane6: HEK-293 whole cell lysate,. Lysates/proteins at 20g per lane.
Western blot analysis of GAPDH in various tissues of different species at dilution of 1:5000.
Lane1 : Rat heart, whole tissue lysate, Lane2: Porcine brain, whole tissue lysate, Lane3: Bovine kidney, whole tissue lysate, Lane4: Guinea pig liver, whole tissue lysate, Lane5: Rabbit pancreas, whole tissue ysate, Lane6: Caprine spleen, whole tissue lysate.
Lysates/proteins at 20g per lane.
Immunofluorescent analysis of 293F cells using anti-GAPDH monoclonal antibody at 1:400 dilution.
Product Name
GAPDH Monoclonal Antibody
Catalog Number
RD86716A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG1
Host
Mouse
Background
GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.
Immunogen Information
Immunogen
Recombinant human GAPDH protein expressed by E.coli
Gene ID
317743
Swissprot
Q5XJ10
Synonyms
aging associated gene 9 proteinBARS-38cb609G3PDHGAPDKNC-NDS6OCASp38 component
Calculated MW
36 kDa
Observed MW
36 kDa
Applications
Reactivity
Human,Mouse,Rat ,Bovine,Caprine,Guinea pig,Rabbit,Porcine,
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:5000-1:10000
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas