GAPDH Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western Blotting of Zebrafish lysates with anti-GAPDH monoclonal antibody at dilution of 1:5000.
Product Name
GAPDH Monoclonal Antibody
Catalog Number
RD86717A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG1
Host
Mouse
Background
GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.
Immunogen Information
Immunogen
Recombinant human GAPDH protein expressed by E.coli
Gene ID
317743
Swissprot
Q5XJ10
Synonyms
aging associated gene 9 proteinBARS-38cb609G3PDHGAPDKNC-NDS6OCASp38 component
Calculated MW
43kDa
Observed MW
43kDa
Applications
Reactivity
Zebrafish
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:2000-1:5000
Concentration
5mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas