GCLC Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD252359A
Western blot analysis of Raw264.7 A549 Hepg2 and Jurkat cell using GCLC Polyclonal Antibody at dilution of 1:800
Immunohistochemistry of paraffin-embedded Human brain tissue using GCLC Polyclonal Antibody at dilution of 1:35(×200)
Product Name
GCLC Polyclonal Antibody
Catalog Number
RD252359A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
Immunogen Information
Immunogen
Fusion protein of human GCLC
Swissprot
P48506
Synonyms
EC 6.3.2.2Gamma ECSGamma glutamylcysteine synthetaseGamma-ECSGamma-glutamylcysteine synthetaseGCLGclcGCSGCS heavy chainGLCLGLCLCGlutamate cysteine ligase catalytic subunitGlutamate--cysteine ligase catalytic subunitGSH1
Calculated MW
73 kDa
Observed MW
Refer to figures
Gene Accession
BC022487
Applications
Reactivity
Human, Mouse, Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:25-1:100, ELISA 1:5000-1:10000
Concentration
0.8 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas