GFP-Tag Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD86713A
Western blot of GFP transfected Zebrafish with anti-GFP-tag at dilution of 1:1000.
Western blot of GFP transfected Zebrafish with anti-GFP-tag at dilution of 1:1000.
Immunofluorescent analysis of GFP transfected 293 cells with GFP-Tag monoclonal antibody at dilution of 1:500.
Immunofluorescent analysis of GFP transfected 293 cells with GFP-Tag monoclonal antibody at dilution of 1:500.
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

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Product Name
GFP-Tag Monoclonal Antibody
Catalog Number
RD86713A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG2a
Host
Mouse

Background

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The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is first isolated from the jellyfish Aequorea victoria and is frequently used as a reporter of expression. GFP is widely used in gene expression, tracing and protein subcellualr localization.

Immunogen Information

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Immunogen
Recombinant GFP protein expressed by E. Coli
Swissprot
P42212
Synonyms
GFPGreen fluorescent protein
Calculated MW
55kDa
Observed MW
55kDa

Applications

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Reactivity
All
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000,IF 1:500-1:2000
Concentration
0.5mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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