Glycerol kinase (GK) Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89746A
Western blot analysis of extracts of various cell lines using Glycerol kinase Polyclonal Antibody at 1:3000 dilution.
Immunohistochemistry of paraffin-embedded human esophageal cancer using Glycerol kinase Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver using Glycerol kinase Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Name
Glycerol kinase (GK) Polyclonal Antibody
Catalog Number
RD89746A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human Glycerol kinase
Gene ID
2710
Swissprot
P32189
Synonyms
GKGK1GKD
Calculated MW
27kDa
Observed MW
28KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas