Dilution
Immunohistochemistry: 1:100-200
Western Blotting: 1:300-10000(Enhanced chemiluminescent detection )
Western Blotting: 1:200-400(DAB detection)
Direct ELISA: 1:5000-20000(TMB detection)
Concentration
0.2 mg/mL(specific antibody purification≥99%),Biotin:Ab=20:1
Storage Buffer
PBS with 50% glycerol, PH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.