GOT1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD83521A
Western blot analysis of extracts of various cell lines using GOT1 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat heart using GOT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using GOT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human colon using GOT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using GOT1 Polyclonal Antibody
Product Name
GOT1 Polyclonal Antibody
Catalog Number
RD83521A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Immunogen Information
Immunogen
Recombinant fusion protein of human GOT1 (NP_002070.1).
Gene ID
2805
Swissprot
P17174
Synonyms
GOT1AST1ASTQTL1GIG18cAspATcCAT
Calculated MW
44 kDa/46 kDa
Observed MW
46 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:10-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas