GOT2 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD84144A
Western blot analysis of extracts of various cell lines using GOT2 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using GOT2 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat kidney using GOT2 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat kidney using GOT2 Polyclonal Antibody at dilution of 1:100 (40x lens).
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Overview

Circle
Product Name
GOT2 Polyclonal Antibody
Catalog Number
RD84144A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human GOT2 (NP_002071.2).
Gene ID
2806
Swissprot
P00505
Synonyms
GOT2KAT4KATIVKYAT4mitAAT
Calculated MW
43 kDa/47 kDa
Observed MW
48 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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