GPBAR1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90276A
Western blot analysis of extracts of Rat liver using GPBAR1 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of 293T cells using GPBAR1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HCT 116 cells using GPBAR1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using GPBAR1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
GPBAR1 Polyclonal Antibody
Catalog Number
RD90276A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.
Immunogen Information
Immunogen
A synthetic peptide of human GPBAR1
Gene ID
151306
Swissprot
Q8TDU6
Synonyms
BG37TGR5M-BARGPCR19GPR131
Calculated MW
35kDa/53kDa/59kDa/60kDa/81kDa/91kDa/93kDa
Observed MW
140KDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.