GPX4 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD86770A
Western blot analysis of various lysates using GPX4 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of various lysates using GPX4 Polyclonal Antibody at 1:1000 dilution.
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
GPX4 Polyclonal Antibody
Catalog Number
RD86770A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human GPX4
Gene ID
2879
Swissprot
P36969
Synonyms
GPX4GPx-4GSHPx-4MCSPPHGPxSMDSsnGPxsnPHGPx
Calculated MW
17kDa
Observed MW
23kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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