GRP78 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86672A
Western blotting with anti-GRP78 polyclonal antibody at dilution of 1:1000. Lane 1: Hela cell lysate, Lane 2: Jurkat cell lysate, lane 3: MCF-7 cell lysate, Lane 4: Mouse lung lysate, Lane 5: Mouse liver lysate, Lane 6: Rat lung lysate, Lane 7: Rat liver lysate, Lane 8: Rat small intestine lysate.
Product Name
GRP78 Polyclonal Antibody
Catalog Number
RD86672A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
GRP78 (Glucose-regulated protein 78 kDa; also BiP and HSPA5) is a 72 kDa member of the heat shock protein 70 family of proteins. Intracellularly, GRP78 is an endoplasmic reticulum chaperone that participates in protein folding; extracellularly, it induces IL-10 production from T cells and interacts with Cripto to block TGF-beta signaling. Human GRP78 precursor is 654 amino acids (aa) in length. It contains an 18 aa signal sequence and a 636 aa mature region that shows a hydantoinase A region (aa 145245) and a C-terminal KDEL motif that is present on intracellular GRP78, but absent on secreted GRP78. There is alternative splicing in the signal sequence (aa 110), and multiple single aa substitituion. Over aa 1654, human GRP78 is more than 97% aa identical to mouse and rat GRP78.
Immunogen Information
Immunogen
Recombinant Human GRP78 protein expressed by E.coli.
Gene ID
3309
Swissprot
P11021
Synonyms
BiPGRP-78HSP70 family protein 5Heat shock protein family A member 5Immunoglobulin heavy chain-binding proteinHSPA5
Calculated MW
26kDa
Observed MW
26kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
1.5mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas