GST-Tag Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86708A
Western blot of four different GST-Tagged fused recombinant proteins with Anti-GST rabbit monoclonal antibody at dilution of 1:1000. Lane 1 : Recombinant GST protein at 6.25ng; Lane 2 : Recombinant GST protein at 12.5ng; Lane 3: Recombinant GST protein at 25ng; Lane 4: Recombinant GST protein at 50ng
Product Name
GST-Tag Monoclonal Antibody
Catalog Number
RD86708A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG
Host
Rabbit
Background
GST , Glutathione-S-transferase, a 26 kDa sequence of 211 amino acids, is another widely used affinity tag that increases solubility of the desired protein. GST tag has affinity for immobilized glutathione and is used for prokaryotic expression more frequently. It can be fused to either the N-terminus or C-terminus of a protein.
Immunogen Information
Immunogen
Recombinant GST protein expressed by E.coli
Synonyms
Glutathione S TransferaseGlutathione S-transferase class-mu 26 kDa isozymeGSTSj26 antigenSjGST
Calculated MW
30kDa
Observed MW
30kDa
Applications
Reactivity
All
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
0.85mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.