GTF3C3 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89074A
Western blot analysis of extracts of various cell lines using GTF3C3 Polyclonal Antibody at1:2000 dilution.
Immunohistochemistry of paraffin-embedded Rat brain using GTF3C3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse brain using GTF3C3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of H9C2 cells using GTF3C3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using GTF3C3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
GTF3C3 Polyclonal Antibody
Catalog Number
RD89074A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is part of the TFIIIC2 complex, which binds to the promoters of small nuclear and cytoplasmic RNA genes in order to recruit RNA polymerase III. The TFIIIC2 complex is composed of six subunits. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Immunogen Information
Immunogen
Recombinant fusion protein of human GTF3C3
Gene ID
9330
Swissprot
Q9Y5Q9
Synonyms
TFIIIC102TFIIICgammaTFiiiC2-102GTF3C3
Observed MW
25kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas