H2AFJ Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD219114A
Western blot analysis of Jurkat cell lysate using H2AFJ Polyclonal Antibody at dilution of 1:800
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using H2AFJ Polyclonal Antibody at dilution of 1:170(×200)
Immunohistochemistry of paraffin-embedded Human breast cancer tissue using H2AFJ Polyclonal Antibody at dilution of 1:170(×200)
Product Name
H2AFJ Polyclonal Antibody
Catalog Number
RD219114A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is located on chromosome 12 and encodes a replication-independent histone that is a variant H2A histone. The protein is divergent at the C-terminus compared to the consensus H2A histone family member. This gene also encodes an antimicrobial peptide with antibacterial and antifungal activity.
Immunogen Information
Immunogen
Fusion protein of human H2AFJ
Swissprot
Q9BTM1
Synonyms
H2A Histone Family Member JHistone H2A.JH2a/J
Calculated MW
14 kDa
Observed MW
Refer to figures
Gene Accession
BC003602
Applications
Reactivity
Human, Mouse, Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:200-1:300, ELISA 1:5000-1:10000
Concentration
1.14 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.