HA-Tag Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86721A
Western blotting with anti-HA-Tag monoclonal antibody at dilution of 1:5000.Lane 1: HA tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate
Immunofluorescent analysis of 293F cells transfected with HA-Tag fusion protein using anti-HA-Tag monoclonal antibody at dilution of 1:800.
IP Result of293F cells transfected with HA-Tag fusion protein,using anti-HA-Tag monoclonal antibody .lane 1: mouse IgG2a Isotype Control,Lane 2:input, Lane 3: anti-HA-Tag mouse monoclonal antibody
1x10^6 CHO cells Transfected with a HA plasmid were stained with 0.2ug Anti-HA tag mAb(RD86721A) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
Product Name
HA-Tag Monoclonal Antibody
Catalog Number
RD86721A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG2a
Host
Mouse
Background
The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.
Immunogen Information
Immunogen
Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin.
Synonyms
HA epitope tagHA1HA2hemagglutininHemagglutinin HA1 chainHemagglutinin HA2 chain
Calculated MW
28kDa
Observed MW
28kDa
Applications
Reactivity
All
Tested Applications
WB, IF,IP,FCM
Conjugation
Unconjugated
Dilution
WB 1:5000-1:10000, IP 3ug/sample, IF 1:400-800,FCM:0.2 ug
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.