HA-Tag Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86722A
Western blotting with anti-HA-Tag rabbit monoclonal antibody at dilution of 1:5000.Lane 1: HA tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate
Immunofluorescent analysis of 293F cells transfected with the HA-GFP using anti-HA-Tag rabbit monoclonal Antibody at 1:2000 dilution.
Product Name
HA-Tag Monoclonal Antibody
Catalog Number
RD86722A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG
Host
Rabbit
Background
The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.
Immunogen Information
Immunogen
Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin.
Synonyms
HA epitope tagHA1HA2hemagglutininHemagglutinin HA1 chainHemagglutinin HA2 chain
Calculated MW
28kDa
Observed MW
28kDa
Applications
Reactivity
All
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:5000-1:10000, IF 1:800-1600
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.